J. John Holbrook

University of Bristol — United Kingdom

Materials Science · Physical Sciences

42h-index5.7kcitations166works0.02yr avg
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Research Topics

Enzyme Structure and Function(66), Protein Structure and Dynamics(62), Amino Acid Enzymes and Metabolism(30), Hemoglobin structure and function(19), Enzyme Catalysis and Immobilization(17)

Publications166 total

Estimating the energetic contribution of hydrogen bonding to the stability of Candida methylica formate dehydrogenase by using double mutant cycle
Biotechnology Letters·2004· 9 cited
Cloning, sequence and expression of the lactate dehydrogenase gene from the human malaria parasite, Plasmodium vivax
Biotechnology Letters·2004· 38 cited
Lactate Dehydrogenase ( <scp>LDH</scp> )
Encyclopedia of Molecular Biology·2002
Mutagenic exploration of the active site of lactate dehydrogenase from Plasmodium falciparum
Biotechnology Letters·2001· 10 cited
Over-production of lactate dehydrogenase from Plasmodium falciparum opens a route to new antimalarials
Biotechnology Letters·2001· 27 cited
The A245K Mutation Exposes Another Stage of the Bacterial <scp>l</scp>-Lactate Dehydrogenase Reaction Mechanism
Biochemistry·2001· 12 cited
Role of glutamate-52 in the mechanism of L-lactate dehydrogenase from Bacillus stearothermophilus
Biotechnology Letters·2001· 3 cited
A single mutation in the NAD-specific formate dehydrogenase from Candida methylica allows the enzyme to use NADP
Biotechnology Letters·2001· 52 cited
Production of an activated form of Bacillus stearothermophilus L-2-hydroxyacid dehydrogenase by directed evolution
Protein Engineering Design and Selection·2000· 25 cited
A general method for relieving substrate inhibition in lactate dehydrogenases
Protein Engineering Design and Selection·1999· 59 cited
A mechanistic and mutagenic investigation of malate dehydrogenase
Bristol Research (University of Bristol)·1999
A general method of domain closure is applied to phosphoglycerate kinase and the result compared with the crystal structure of a closed conformation of the enzyme
Proteins Structure Function and Bioinformatics·1998· 11 cited
A general method of domain closure is applied to phosphoglycerate kinase and the result compared with the crystal structure of a closed conformation of the enzyme
Proteins Structure Function and Bioinformatics·1998
Correlation of the enzyme activities ofBacillus stearothermophilus lactate dehydrogenase on three substrates with the results of molecular dynamics/energy minimization conformational searching
Proteins Structure Function and Bioinformatics·1997· 4 cited
Correlation of the enzyme activities of Bacillus stearothermophilus lactate dehydrogenase on three substrates with the results of molecular dynamics/energy minimization conformational searching
Proteins Structure Function and Bioinformatics·1997
A model of Plasmodium falciparum lactate dehydrogenase and its implications for the design of improved antimalarials and the enhanced detection of parasitaemia
Protein Engineering Design and Selection·1997· 39 cited
D‐2‐Hydroxy‐4‐Methylvalerate Dehydrogenase from <i>Lactobacillus Delbrueckii</i> Subsp. <i>Bulgaricus</i>— II. Mutagenic analysis of catalytically important residues
European Journal of Biochemistry·1997· 21 cited
D‐2‐Hydroxy‐4‐Methylvalerate Dehydrogenase from <i>Lactobacillus Delbrueckii</i> Subsp. <i>Bulgaricus</i>— I. Kinetic Mechanism and pH Dependence of Kinetic Parameters, Coenzyme Binding and Substrate Inhibition
European Journal of Biochemistry·1997· 10 citedOpen Access
Protein engineering tests of a homology model of Plasmodium falciparum lactate dehydrogenase
Protein Engineering Design and Selection·1997· 23 citedOpen Access
Removal of substrate inhibition in a lactate dehydrogenase from human muscle by a single residue change
FEBS Letters·1996· 62 citedOpen Access
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Frequent Co-authors

Anthony R. Clarke(21), Tony Atkinson(18), William Chia(9), David A. Barstow(9), Dale B. Wigley(8), Richard B. Sessions(6), Keith Hart(5), David Halsall(4), Adam Waldman(4), Alan R. Clarke(4), Helen M. Wilks(3), Cameron R. Dunn(3), Hilary Muirhead(3), Rosemary A. Staniforth(3), Steven G. Burston(3), Kathleen M. Moreton(3), Corinne J. Smith(3), R. Jeckel(3), Raymond Feeney(2), Graham S. Jackson(2)