Kai Scheffler

Thermo Fisher Scientific (Germany) — Germany

Biochemistry, Genetics and Molecular Biology · Life Sciences

12h-index637citations24works1.02yr avg
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Research Topics

Protein purification and stability(16), Monoclonal and Polyclonal Antibodies Research(12), Viral Infectious Diseases and Gene Expression in Insects(8), Mass Spectrometry Techniques and Applications(6), Advanced Proteomics Techniques and Applications(4)

Publications24 total

Improved Mass Accuracy and Precision for Multi-Attribute Methods Using a New Internally Calibrated High Resolution Orbitrap Mass Detector
Analytical Chemistry·2024· 2 cited
Characterization of an IgG1 monoclonal antibody oxidation variants at intact, subunit and peptide levels by High Resolution Accurate Mass (HRAM) mass spectrometry
2024Open Access
Multi-Attribute Method (MAM): An Emerging Analytical Workflow for Biopharmaceutical Characterization, Batch Release and cGMP Purity Testing at the Peptide and Intact Protein Level
Critical Reviews in Analytical Chemistry·2023· 24 citedOpen Access
Proteomic Profiling of IgG1 Producing CHO Cells Using LC/LC-SPS-MS3: The Effects of Bioprocessing Conditions on Productivity and Product Quality
Frontiers in Bioengineering and Biotechnology·2021· 13 citedOpen Access
Tracking the Behavior of Monoclonal Antibody Product Quality Attributes Using a Multi-Attribute Method Workflow
Journal of the American Society for Mass Spectrometry·2021· 54 citedOpen Access
Optimisation of the use of sliding window deconvolution for comprehensive characterisation of trastuzumab and adalimumab charge variants by native high resolution mass spectrometry
European Journal of Pharmaceutics and Biopharmaceutics·2020· 21 citedOpen Access
Cracking Proteoform Complexity of Ovalbumin with Anion-Exchange Chromatography–High-Resolution Mass Spectrometry under Native Conditions
Journal of Proteome Research·2019· 35 citedOpen Access
Comprehensive characterisation of the heterogeneity of adalimumab via charge variant analysis hyphenated on-line to native high resolution Orbitrap mass spectrometry
mAbs·2018· 91 citedOpen Access
Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals
Nature Communications·2018· 116 citedOpen Access
Charge Variant Analysis of Monoclonal Antibodies Using Direct Coupled pH Gradient Cation Exchange Chromatography to High-Resolution Native Mass Spectrometry
Analytical Chemistry·2018· 127 cited
Monoclonal antibody sequence assessment using a hybrid quadrupole-Orbitrap mass spectrometer
Analytical Methods·2018· 5 cited
Cysteine-SILAC Mass Spectrometry Enabling the Identification and Quantitation of Scrambled Interchain Disulfide Bonds: Preservation of Native Heavy-Light Chain Pairing in Bispecific IgGs Generated by Controlled Fab-arm Exchange
Analytical Chemistry·2017· 13 cited
Intact Mass Analysis of Complex Therapeutic Proteins under Native Conditions
Genetic Engineering & Biotechnology News·2017
High resolution top-down experimental strategies on the Orbitrap platform
Journal of Proteomics·2017· 20 cited
Full Characterization of Heterogeneous Antibody Samples under Denaturing and Native Conditionson a Hybrid Quadrupole-Orbitrap Mass Spectrometer
World Academy of Science, Engineering and Technology, International Journal of Pharmacological and Pharmaceutical Sciences·2017
Native mass spectrometry for the revelation of highly complex glycosylation patterns in biopharmaceuticals
2017
A Top to Bottom Approach for In-Depth Characterization of Therapeutic Monoclonal Antibodies
2015
Rapid Characterization of Recombinant Therapeutic Monoclonal Antibodies with HPLC-MS
2014
High Resolution ESI-Orbitrap MS Measurement of Intact PEGylated rhG-CSF and Oxidation Site Assignment of Stressed Variants
2014
Top-Down Proteomics by Means of Orbitrap Mass Spectrometry
Methods in molecular biology·2014· 9 cited
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Frequent Co-authors

Jonathan Bones(9), Christian G. Huber(7), Ken Cook(6), Sara Carillo(5), Florian Füssl(4), Ines Forstenlehner(4), Eugen Damoc(4), Amy Farrell(3), Johann Holzmann(3), Silvia Millán‐Martín(3), Martin Samonig(3), Therese Wohlschlager(2), Stefan Senn(2), Craig Jakes(2), Wolfgang Wieder(2), Hansjörg Toll(2), Jennifer N. Sutton(2), Rosa Viner(2), Simon Cubbon(2), Aaron O. Bailey(2)